Regulatory

Part:BBa_K3844003:Design

Designed by: Anya Sabhnani   Group: iGEM21_DNHS_SanDiego_CA   (2021-10-21)


NR1D2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 435
    Illegal PstI site found at 619
    Illegal PstI site found at 1486
    Illegal PstI site found at 1582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 435
    Illegal PstI site found at 619
    Illegal PstI site found at 1486
    Illegal PstI site found at 1582
    Illegal NotI site found at 1130
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 435
    Illegal PstI site found at 619
    Illegal PstI site found at 1486
    Illegal PstI site found at 1582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 435
    Illegal PstI site found at 619
    Illegal PstI site found at 1486
    Illegal PstI site found at 1582
    Illegal NgoMIV site found at 663
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our project requires for the transcription factor to be dependent on the presence of VOCs. When researching the design of trimethylbenzene, the VOC that this transcription factor binds to, we needed to make sure that, hypothetically, everything would work based on our model. After checking through extensive research that our design would fit with this transcription factor, we chose it.



Source

DNA binding sites

References